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The tender-clipped sequences that overhang the breakpoint are collapsed right into a consensus sequence using a several sequence alignment (MSA) furnished during the seqAn library [27]. Wham applies three filters into the consensus sequences. Breakpoints are usually not documented in situations where by consensus sequences are shorter than ten bp o
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Wham is suitable for paired-close Illumina libraries with standard insert measurements (~300bp-500bp). Wham integrates mate-pair mapping, break up read mapping, gentle-clipping, substitute alignment and consensus sequence primarily based proof to predict SV breakpoints with solitary-nucleotide precision. Wham generates a merged pileup (catalog of r
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